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Analytical Instrument Systems Inc
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Ohmic Instruments Inc
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Spectramed Inc
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DigiTec Corporation
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Ocean Insight
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ThyssenKrupp Industrial Solutions AG
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Mokon Inc
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ANSYS inc
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Persistent Systems LTD
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Formech Inc
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Simio LLC
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CH Instruments
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Image Search Results
Journal: Nanomaterials
Article Title: Intracranial Gene Delivery Mediated by Albumin-Based Nanobubbles and Low-Frequency Ultrasound
doi: 10.3390/nano14030285
Figure Lengend Snippet: Schematic representation of ultrasound treatment of albumin-based NBs or sonoporation in 96-well plates. ( A ) Sonication treatment of NBs by applying ultrasound to the medium. ( B – F ) Sonoporation method. ( B ) Incubation medium is removed from the well (white arrow) of a 96-well plate seeded with malignant glioma cells (U-87 MG). ( C ) Each well is filled with medium containing NBs (white arrow). ( D ) Transfection by ultrasound. pDNA or mRNA is transferred into the cytoplasm (white arrow). ( E ) Aspiration of sonicated medium (white arrow 1) and ( E ) addition of fresh incubation medium (white arrow 2). ( F ) After 24 or 48 h of incubation, the supernatant was collected for reporter assay. ( G ) Arrangement of wells with seeded cells (indicated by color) on a 96-well plate. NBs: nanobubbles.
Article Snippet: The ultrasound irradiation device used had a driving frequency of 47 kHz and an intensity of 1.29 W/cm 2 measured by a radiation force balance using an
Techniques: Sonication, Incubation, Transfection, Reporter Assay
Journal: Nanomaterials
Article Title: Intracranial Gene Delivery Mediated by Albumin-Based Nanobubbles and Low-Frequency Ultrasound
doi: 10.3390/nano14030285
Figure Lengend Snippet: Overview of in vitro ultrasound imaging of albumin-based NBs. ( A ) LoFreqUS irradiation into A-NBs solution. ( B ) Acoustic evaluation using a diagnostic US imaging system. NBs: nanobubbles, US: ultrasound.
Article Snippet: The ultrasound irradiation device used had a driving frequency of 47 kHz and an intensity of 1.29 W/cm 2 measured by a radiation force balance using an
Techniques: In Vitro, Imaging, Irradiation, Diagnostic Assay
Journal: Nanomaterials
Article Title: Intracranial Gene Delivery Mediated by Albumin-Based Nanobubbles and Low-Frequency Ultrasound
doi: 10.3390/nano14030285
Figure Lengend Snippet: Physical properties of NBs at different ultrasound exposure times. ( A ) Size distribution of NBs on NTA. ( B ) FCM measurement NBs size distribution and concentration after 40-fold dilution. Yellow or gray shaded area indicates NBs size less than 200 nm or more than 500 nm, respectively. NBs: nanobubbles.
Article Snippet: The ultrasound irradiation device used had a driving frequency of 47 kHz and an intensity of 1.29 W/cm 2 measured by a radiation force balance using an
Techniques: Concentration Assay
Journal: Nanomaterials
Article Title: Intracranial Gene Delivery Mediated by Albumin-Based Nanobubbles and Low-Frequency Ultrasound
doi: 10.3390/nano14030285
Figure Lengend Snippet: Visualization of albumin-based NBs using ultrasound diagnostic equipment. ( A ) Image of NBs solution before LoFreqUS irradiation. ( B ) Image of NBs solution after LoFreqUS irradiation. ( C ) Image of control solution. NBs: nanobubbles.
Article Snippet: The ultrasound irradiation device used had a driving frequency of 47 kHz and an intensity of 1.29 W/cm 2 measured by a radiation force balance using an
Techniques: Diagnostic Assay, Irradiation, Control
Journal: Nanomaterials
Article Title: Intracranial Gene Delivery Mediated by Albumin-Based Nanobubbles and Low-Frequency Ultrasound
doi: 10.3390/nano14030285
Figure Lengend Snippet: Cell viability after sonication. Cell viability with and without NBs and after different ultrasound exposure times, data are presented as mean ± standard error of the mean (s.e.m.). Statistical significance was assessed by unpaired t -test (n.s. not significant) ( n = 4). NBs: nanobubbles.
Article Snippet: The ultrasound irradiation device used had a driving frequency of 47 kHz and an intensity of 1.29 W/cm 2 measured by a radiation force balance using an
Techniques: Sonication
Journal: Nanomaterials
Article Title: Intracranial Gene Delivery Mediated by Albumin-Based Nanobubbles and Low-Frequency Ultrasound
doi: 10.3390/nano14030285
Figure Lengend Snippet: Intracranial luciferase expression in mice after intrathecal injection of pDNA. ( A ) Representative IVIS images of mice one day after sonoporation. ( B ) Quantification of luciferase expression of the cranial region over time based on IVIS images. Data are presented as mean ± standard error of the mean (s.e.m.). Statistical significance was determined using a one-way ANOVA (** p < 0.01, *** p < 0.001), NBs+US− ( n = 4), NBs−US+ ( n = 6), NBs+US+ ( n = 5). NBs: nanobubbles, US: ultrasound.
Article Snippet: The ultrasound irradiation device used had a driving frequency of 47 kHz and an intensity of 1.29 W/cm 2 measured by a radiation force balance using an
Techniques: Luciferase, Expressing, Injection
Journal: Nanomaterials
Article Title: Intracranial Gene Delivery Mediated by Albumin-Based Nanobubbles and Low-Frequency Ultrasound
doi: 10.3390/nano14030285
Figure Lengend Snippet: Intracranial Luciferase expression in mice after intrathecal injection of mRNA. ( A ) Representative IVIS images of mice one day after sonoporation. ( B ) Quantification of luciferase expression of the cranial region on IVIS images. Data are presented as mean ± standard error of the mean (s.e.m.). Statistical significance was determined using a one-way ANOVA (n.s. not significant), NBs+US− ( n = 3), NBs−US+ ( n = 4), NBs+US+ ( n = 4). NBs: nanobubbles, US: ultrasound.
Article Snippet: The ultrasound irradiation device used had a driving frequency of 47 kHz and an intensity of 1.29 W/cm 2 measured by a radiation force balance using an
Techniques: Luciferase, Expressing, Injection